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Overview

Product name
Description

Rabbit monoclonal [SP13] to Calretinin
Host species

Rabbit
Tested applications
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant full length protein within Mouse Calretinin. The exact sequence is proprietary.
Positive control
- Human mesothelioma for Immunohistochemistry. IHC-Fr: Mouse and rat cerebrum tissue
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.50
Preservative: 0.1% Sodium azide
Constituents: Tissue culture supernatant, Tris buffered saline, 1% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

SP13
Isotype

IgG
Research areas
- Neuroscience
- Neurotransmission
- Calcium Signaling
- Calcium Binding Proteins

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P	(1)	1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr		1/30.

Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/30.

Target

Function

Calretinin is a calcium-binding protein which is abundant in auditory neurons.
Tissue specificity

Brain.
Sequence similarities

Belongs to the calbindin family.
Contains 6 EF-hand domains.
Information by UniProt
Database links
Alternative names
- 29 kDa calbindin antibody
- CAB 29 antibody
- CAB29 antibody
- CAL 2 antibody
- CAL2 antibody
- CALB 2 antibody
- CALB2 antibody
- CALB2_HUMAN antibody
- Calbindin 2 29kDa antibody
- Calbindin 2 antibody
- Calbindin D29K antibody
- Calbindin2 antibody
- Calretinin antibody
- CR antibody
see all

Images

Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Frozen sections) - Anti-Calretinin antibody [SP13] (ab16694)

Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calretinin antibody [SP13] (ab16694)

Ab16694 at a dilution of 1/100, staining Calretinin in formalin fixed paraffin embedded human mesothelioma tissue section by Immunohistochemistry.

Protocols

Datasheets and documents

SDS download
Datasheet download

References (9)

ab16694 has been referenced in 9 publications.

Pruett Net al. Normal mesothelial cell lines newly derived from human pleural biopsy explants. Am J Physiol Lung Cell Mol Physiol 319:L652-L660 (2020). PubMed: 32726133
Murayama AYet al. The polymicrogyria-associated GPR56 promoter preferentially drives gene expression in developing GABAergic neurons in common marmosets. Sci Rep 10:21516 (2020). PubMed: 33299078
Sakaguchi Het al. Self-Organized Synchronous Calcium Transients in a Cultured Human Neural Network Derived from Cerebral Organoids. Stem Cell Reports 13:458-473 (2019). PubMed: 31257131
Ohara Yet al. Connective tissue growth factor-specific monoclonal antibody inhibits growth of malignant mesothelioma in an orthotopic mouse model. Oncotarget 9:18494-18509 (2018). PubMed: 29719620
Yin Met al. Gremlin-1 is a key regulator of the invasive cell phenotype in mesothelioma. Oncotarget 8:98280-98297 (2017). PubMed: 29228689
Roulois Det al. Characterization of preneoplastic and neoplastic rat mesothelial cell lines: the involvement of TETs, DNMTs, and 5-hydroxymethylcytosine. Oncotarget 7:34664-87 (2016). PubMed: 27129173
Aierken Det al. Rat model demonstrates a high risk of tremolite but a low risk of anthophyllite for mesothelial carcinogenesis. Nagoya J Med Sci 76:149-60 (2014). PubMed: 25130001
Lin Bet al. Forebrain ischemia triggers GABAergic system degeneration in substantia nigra at chronic stages in rats. Cardiovasc Psychiatry Neurol 2010:506952 (2010). IHC ; Rat . PubMed: 20981346
Kuwabara Tet al. Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis. Nat Neurosci 12:1097-105 (2009). ICC/IF ; Rat . PubMed: 19701198